diff --git a/sdk/spring/CHANGELOG.md b/sdk/spring/CHANGELOG.md
index f54a01d8607d..2a1a99ff3300 100644
--- a/sdk/spring/CHANGELOG.md
+++ b/sdk/spring/CHANGELOG.md
@@ -13,6 +13,10 @@ This section includes changes in `spring-cloud-azure-autoconfigure` module.
 - Add ConnectionDetails for ServiceBus. [#44019](https://github.com/Azure/azure-sdk-for-java/pull/44019).
 - Add ConnectionDetails for EventHubs. [#47926](https://github.com/Azure/azure-sdk-for-java/pull/47926).
 
+#### Bugs Fixed
+
+- Fixed `KeyVaultJcaProvider` being registered as the highest-priority JCA security provider, which overrides standard JCA services (`KeyManagerFactory.SunX509`, `Signature` algorithms) and breaks mTLS with standard keystores (JKS, PKCS12). The provider is now added at the end of the provider list, allowing JCA's delayed provider selection to route `KeyVaultPrivateKey` signing operations to the KeyVault implementations without interfering with standard SSL/TLS operations. [#48183](https://github.com/Azure/azure-sdk-for-java/issues/48183)
+
 ### Spring Cloud Azure Docker Compose
 
 This section includes changes in `spring-cloud-azure-docker-compose` module.
diff --git a/sdk/spring/spring-cloud-azure-autoconfigure/src/main/java/com/azure/spring/cloud/autoconfigure/implementation/keyvault/jca/AzureKeyVaultSslBundleRegistrar.java b/sdk/spring/spring-cloud-azure-autoconfigure/src/main/java/com/azure/spring/cloud/autoconfigure/implementation/keyvault/jca/AzureKeyVaultSslBundleRegistrar.java
index 2a94664e3db6..10521f66f533 100644
--- a/sdk/spring/spring-cloud-azure-autoconfigure/src/main/java/com/azure/spring/cloud/autoconfigure/implementation/keyvault/jca/AzureKeyVaultSslBundleRegistrar.java
+++ b/sdk/spring/spring-cloud-azure-autoconfigure/src/main/java/com/azure/spring/cloud/autoconfigure/implementation/keyvault/jca/AzureKeyVaultSslBundleRegistrar.java
@@ -137,7 +137,7 @@ private KeyStore initilizeKeyVaultKeyStore(String storeName,
         configureJcaKeyStoreSystemProperties(jcaVaultProperties, keyStoreProperties, resourceLoader);
         if (providerConfigured.compareAndSet(false, true)) {
             Security.removeProvider(KeyVaultJcaProvider.PROVIDER_NAME);
-            Security.insertProviderAt(new KeyVaultJcaProvider(), 1);
+            Security.addProvider(new KeyVaultJcaProvider());
         }
         KeyStore azureKeyVaultKeyStore;
         try {
diff --git a/sdk/spring/spring-cloud-azure-autoconfigure/src/test/java/com/azure/spring/cloud/autoconfigure/implementation/keyvault/jca/AzureKeyVaultSslBundleRegistrarTests.java b/sdk/spring/spring-cloud-azure-autoconfigure/src/test/java/com/azure/spring/cloud/autoconfigure/implementation/keyvault/jca/AzureKeyVaultSslBundleRegistrarTests.java
index ba2539ab7c9c..2d1a99583046 100644
--- a/sdk/spring/spring-cloud-azure-autoconfigure/src/test/java/com/azure/spring/cloud/autoconfigure/implementation/keyvault/jca/AzureKeyVaultSslBundleRegistrarTests.java
+++ b/sdk/spring/spring-cloud-azure-autoconfigure/src/test/java/com/azure/spring/cloud/autoconfigure/implementation/keyvault/jca/AzureKeyVaultSslBundleRegistrarTests.java
@@ -3,8 +3,10 @@
 
 package com.azure.spring.cloud.autoconfigure.implementation.keyvault.jca;
 
+import com.azure.security.keyvault.jca.KeyVaultJcaProvider;
 import com.azure.spring.cloud.autoconfigure.implementation.keyvault.jca.properties.AzureKeyVaultJcaProperties;
 import com.azure.spring.cloud.autoconfigure.implementation.keyvault.jca.properties.AzureKeyVaultSslBundleProperties;
+import org.junit.jupiter.api.AfterEach;
 import org.junit.jupiter.api.Test;
 import org.junit.jupiter.api.extension.ExtendWith;
 import org.junit.jupiter.api.parallel.Isolated;
@@ -19,6 +21,7 @@
 import org.springframework.util.ClassUtils;
 
 import java.security.KeyStore;
+import java.security.Security;
 import java.util.Arrays;
 
 import static org.junit.jupiter.api.Assertions.assertTrue;
@@ -33,6 +36,11 @@
 @ExtendWith({OutputCaptureExtension.class})
 class AzureKeyVaultSslBundleRegistrarTests {
 
+    @AfterEach
+    void cleanupProvider() {
+        Security.removeProvider(KeyVaultJcaProvider.PROVIDER_NAME);
+    }
+
     @Test
     void noJcaProviderOnClassPath(CapturedOutput capturedOutput) {
         AzureKeyVaultSslBundleRegistrar registrar = new AzureKeyVaultSslBundleRegistrar(new AzureKeyVaultJcaProperties(), new AzureKeyVaultSslBundleProperties());
@@ -200,4 +208,42 @@ void registerMultipleSslBundles(CapturedOutput capturedOutput) {
             });
         }
     }
+
+    @Test
+    void keyVaultProviderNotInsertedAtHighestPriority() {
+        AzureKeyVaultJcaProperties jcaProperties = new AzureKeyVaultJcaProperties();
+        AzureKeyVaultSslBundleProperties sslBundleProperties = new AzureKeyVaultSslBundleProperties();
+        AzureKeyVaultSslBundleRegistrar registrar = new AzureKeyVaultSslBundleRegistrar(jcaProperties, sslBundleProperties);
+        registrar.setResourceLoader(new DefaultResourceLoader());
+        SslBundleRegistry registry = Mockito.mock(SslBundleRegistry.class);
+
+        try (MockedStatic<KeyStore> keyStoreMockedStatic = mockStatic(KeyStore.class)) {
+            KeyStore keyStore = Mockito.mock(KeyStore.class);
+            keyStoreMockedStatic.when(() -> KeyStore.getInstance(KeyVaultJcaProvider.PROVIDER_NAME)).thenReturn(keyStore);
+
+            String keyvaultName = "keyvault1";
+            AzureKeyVaultJcaProperties.JcaVaultProperties jcaVaultProperties = new AzureKeyVaultJcaProperties.JcaVaultProperties();
+            jcaVaultProperties.setEndpoint("https://test.vault.azure.net/");
+            jcaProperties.getVaults().put(keyvaultName, jcaVaultProperties);
+            AzureKeyVaultSslBundleProperties.KeyVaultSslBundleProperties bundleProperties = new AzureKeyVaultSslBundleProperties.KeyVaultSslBundleProperties();
+            bundleProperties.getTruststore().setKeyvaultRef(keyvaultName);
+            sslBundleProperties.getKeyvault().put("testBundle", bundleProperties);
+
+            registrar.registerBundles(registry);
+
+            // Verify the KeyVault JCA provider is NOT inserted at position 1 (highest priority),
+            // to avoid interfering with standard SSL/TLS operations and breaking mTLS.
+            java.security.Provider[] providers = Security.getProviders();
+            int providerPosition = -1;
+            for (int i = 0; i < providers.length; i++) {
+                if (KeyVaultJcaProvider.PROVIDER_NAME.equals(providers[i].getName())) {
+                    providerPosition = i + 1; // 1-indexed position
+                    break;
+                }
+            }
+            assertTrue(providerPosition > 1, "KeyVaultJcaProvider must not be inserted at position 1 "
+                + "to avoid overriding standard JCA services like KeyManagerFactory.SunX509 and Signature algorithms, "
+                + "which would break mTLS with standard keystores.");
+        }
+    }
 }