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> Servant N, Varoquaux N, Lajoie BR, Viara E, Chen C, Vert JP, Dekker J, Heard E, Barillot E. Genome Biology 2015, 16:259 doi: [10.1186/s13059-015-0831-x](https://dx.doi.org/10.1186/s13059-015-0831-x)
> Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
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Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string.
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> - The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
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> - Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
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> - If you are using `singularity`, please use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
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> - If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
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> * The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
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> * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
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> * If you are using `singularity`, please use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
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> * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
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4. Start running your own analysis!
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## Citations
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If you use nf-core/hic for your analysis, please cite it using the following doi: doi: [10.5281/zenodo.2669513](https://doi.org/10.5281/zenodo.2669513)
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If you use nf-core/hic for your analysis, please cite it using the following doi: doi: [10.5281/zenodo.2669513](https://doi.org/10.5281/zenodo.2669513)
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An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
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*[Compartments calling](#compartments-calling)
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*[TADs calling](#tads-calling)
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*[MultiQC](#multiqc) - aggregate report and quality controls, describing
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results of the whole pipeline
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results of the whole pipeline
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*[Export](#exprot) - additionnal export for compatibility with downstream
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analysis tool and visualization
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analysis tool and visualization
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## HiC-Pro
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Invalid pairs are classified as follow:
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* Dangling end, i.e. unligated fragments (both reads mapped on the same
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restriction fragment)
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restriction fragment)
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* Self circles, i.e. fragments ligated on themselves (both reads mapped on the
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same restriction fragment in inverted orientation)
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same restriction fragment in inverted orientation)
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* Religation, i.e. ligation of juxtaposed fragments
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* Filtered pairs, i.e. any pairs that do not match the filtering criteria on
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inserts size, restriction fragments size
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inserts size, restriction fragments size
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* Dumped pairs, i.e. any pairs for which we were not able to reconstruct the
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ligation product.
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ligation product.
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Only valid pairs involving two different restriction fragments are used to
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build the contact maps.
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<detailsmarkdown="1">
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<summary>Output files</summary>
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-`multiqc/`
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-`multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
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-`multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
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-`multiqc_plots/`: directory containing static images from the report in various formats.
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*`multiqc/`
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*`multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
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*`multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
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*`multiqc_plots/`: directory containing static images from the report in various formats.
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</details>
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<detailsmarkdown="1">
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<summary>Output files</summary>
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-`pipeline_info/`
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- Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
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- Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline.
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- Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
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*`pipeline_info/`
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* Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
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* Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline.
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* Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
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## Core Nextflow arguments
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> **NB:** These options are part of Nextflow and use a _single_ hyphen
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(pipeline parameters use a double-hyphen).
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> (pipeline parameters use a double-hyphen).
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### `-profile`
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Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. When using Biocontainers, most of these software packaging methods pull Docker containers from quay.io e.g [FastQC](https://quay.io/repository/biocontainers/fastqc) except for Singularity which directly downloads Singularity images via https hosted by the [Galaxy project](https://depot.galaxyproject.org/singularity/) and Conda which downloads and installs software locally from [Bioconda](https://bioconda.github.io/).
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> We highly recommend the use of Docker or Singularity containers for full
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pipeline reproducibility, however when this is not possible, Conda is also supported.
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> pipeline reproducibility, however when this is not possible, Conda is also supported.
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The pipeline also dynamically loads configurations from
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expect all software to be
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installed and available on the `PATH`. This is _not_ recommended.
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-`docker`
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- A generic configuration profile to be used with [Docker](https://docker.com/)
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-`singularity`
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- A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
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-`podman`
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- A generic configuration profile to be used with [Podman](https://podman.io/)
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-`shifter`
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- A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
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-`charliecloud`
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- A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
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-`conda`
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- A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
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-`test`
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- A profile with a complete configuration for automated testing
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- Includes links to test data so needs no other parameters
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*`docker`
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* A generic configuration profile to be used with [Docker](https://docker.com/)
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*`singularity`
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* A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/)
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*`podman`
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* A generic configuration profile to be used with [Podman](https://podman.io/)
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*`shifter`
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* A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
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*`charliecloud`
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* A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
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*`conda`
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* A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
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*`test`
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* A profile with a complete configuration for automated testing
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* Includes links to test data so needs no other parameters
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### `-resume`
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2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags)
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3. Create the custom config accordingly:
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- For Docker:
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* For Docker:
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```nextflow
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process {
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}
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```
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- For Singularity:
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* For Singularity:
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```nextflow
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process {
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}
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```
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- For Conda:
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* For Conda:
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```nextflow
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process {
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1. The path must be enclosed in quotes
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2. The path must have at least one `*` wildcard character
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3. When using the pipeline with paired end data, the path must use `{1,2}`
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notation to specify read pairs.
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notation to specify read pairs.
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If left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`
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This parameter allows to automatically set the `--restriction_site` and
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`--ligation_site` parameter according to the restriction enzyme you used.
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Available keywords are 'hindiii', 'dpnii', 'mboi', 'arima'.
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Available keywords are 'hindiii', 'dpnii', 'mboi', 'arima'.
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