diff --git a/providers/amazon/src/airflow/providers/amazon/aws/operators/glue.py b/providers/amazon/src/airflow/providers/amazon/aws/operators/glue.py
index 3d46aecb32e0b..30077380eb641 100644
--- a/providers/amazon/src/airflow/providers/amazon/aws/operators/glue.py
+++ b/providers/amazon/src/airflow/providers/amazon/aws/operators/glue.py
@@ -433,7 +433,7 @@ def __init__(
     ):
         super().__init__(**kwargs)
         self.name = name
-        self.ruleset = ruleset.strip()
+        self.ruleset = ruleset
         self.description = description
         self.update_rule_set = update_rule_set
         self.data_quality_ruleset_kwargs = data_quality_ruleset_kwargs or {}
@@ -449,6 +449,8 @@ def validate_inputs(self) -> None:
                 raise AttributeError("Target table must have DatabaseName and TableName")
 
     def execute(self, context: Context):
+        # ruleset is a template field; strip the rendered value here, not in __init__.
+        self.ruleset = self.ruleset.strip()
         self.validate_inputs()
 
         config = {
diff --git a/providers/amazon/tests/unit/amazon/aws/operators/test_glue.py b/providers/amazon/tests/unit/amazon/aws/operators/test_glue.py
index 230193feeb42b..99d22e3bb5e47 100644
--- a/providers/amazon/tests/unit/amazon/aws/operators/test_glue.py
+++ b/providers/amazon/tests/unit/amazon/aws/operators/test_glue.py
@@ -17,6 +17,7 @@
 from __future__ import annotations
 
 from collections.abc import Generator
+from datetime import datetime
 from typing import TYPE_CHECKING
 from unittest import mock
 
@@ -25,6 +26,7 @@
 from boto3 import client
 from moto import mock_aws
 
+from airflow.models.dag import DAG
 from airflow.providers.amazon.aws.hooks.glue import GlueDataQualityHook, GlueJobHook
 from airflow.providers.amazon.aws.hooks.s3 import S3Hook
 from airflow.providers.amazon.aws.links.glue import GlueJobRunDetailsLink
@@ -813,6 +815,28 @@ def test_execute_create_rule(self, glue_data_quality_mock_conn):
             Ruleset=self.RULE_SET,
         )
 
+    @mock.patch.object(GlueDataQualityHook, "conn")
+    def test_execute_strips_rendered_ruleset(self, glue_data_quality_mock_conn):
+        # ruleset is a template field; execute strips the rendered value (rendering can add whitespace).
+        with DAG("glue_dq_strip", schedule=None, start_date=datetime(2020, 1, 1)) as dag:
+            self.operator = GlueDataQualityOperator(
+                task_id="create_data_quality_ruleset",
+                name=self.RULE_SET_NAME,
+                ruleset="{{ params.rules }}",
+                dag=dag,
+            )
+        self.operator.defer = mock.MagicMock()
+        self.operator.render_template_fields({"params": {"rules": f"  {self.RULE_SET}  "}})
+        assert self.operator.ruleset == f"  {self.RULE_SET}  "
+
+        self.operator.execute({})
+
+        glue_data_quality_mock_conn.create_data_quality_ruleset.assert_called_once_with(
+            Description="AWS Glue Data Quality Rule Set With Airflow",
+            Name=self.RULE_SET_NAME,
+            Ruleset=self.RULE_SET,
+        )
+
     @mock.patch.object(GlueDataQualityHook, "conn")
     def test_execute_create_rule_should_fail_if_rule_already_exists(self, glue_data_quality_mock_conn):
         self.operator = GlueDataQualityOperator(
diff --git a/scripts/ci/prek/validate_operators_init_exemptions.txt b/scripts/ci/prek/validate_operators_init_exemptions.txt
index 01fd5bc56dbb7..b6cbde980d002 100644
--- a/scripts/ci/prek/validate_operators_init_exemptions.txt
+++ b/scripts/ci/prek/validate_operators_init_exemptions.txt
@@ -14,7 +14,6 @@ providers/amazon/src/airflow/providers/amazon/aws/operators/dms.py::DmsModifyTas
 providers/amazon/src/airflow/providers/amazon/aws/operators/dms.py::DmsStartReplicationOperator
 providers/amazon/src/airflow/providers/amazon/aws/operators/ecs.py::EcsRunTaskOperator
 providers/amazon/src/airflow/providers/amazon/aws/operators/emr.py::EmrAddStepsOperator
-providers/amazon/src/airflow/providers/amazon/aws/operators/glue.py::GlueDataQualityOperator
 providers/amazon/src/airflow/providers/amazon/aws/operators/neptune.py::NeptuneStartDbClusterOperator
 providers/amazon/src/airflow/providers/amazon/aws/operators/neptune.py::NeptuneStopDbClusterOperator
 providers/amazon/src/airflow/providers/amazon/aws/operators/s3.py::S3DeleteObjectsOperator