diff --git a/packages/google-auth/google/auth/_agent_identity_utils.py b/packages/google-auth/google/auth/_agent_identity_utils.py
index 299c56ccbfde..8a1eddbe1cd3 100644
--- a/packages/google-auth/google/auth/_agent_identity_utils.py
+++ b/packages/google-auth/google/auth/_agent_identity_utils.py
@@ -22,9 +22,7 @@
 import time
 from urllib.parse import quote, urlparse
 
-from google.auth import environment_vars
-from google.auth import exceptions
-
+from google.auth import environment_vars, exceptions
 
 _LOGGER = logging.getLogger(__name__)
 
@@ -37,6 +35,8 @@
 _AGENT_IDENTITY_SPIFFE_TRUST_DOMAIN_PATTERNS = [
     r"^agents\.global\.org-\d+\.system\.id\.goog$",
     r"^agents\.global\.proj-\d+\.system\.id\.goog$",
+    r"^agents-nonprod\.global\.org-\d+\.system\.id\.goog$",
+    r"^agents-nonprod\.global\.proj-\d+\.system\.id\.goog$",
 ]
 
 _WELL_KNOWN_CERT_PATH = "/var/run/secrets/workload-spiffe-credentials/certificates.pem"
diff --git a/packages/google-auth/tests/test_agent_identity_utils.py b/packages/google-auth/tests/test_agent_identity_utils.py
index b2e690c7465a..f74bdad9e475 100644
--- a/packages/google-auth/tests/test_agent_identity_utils.py
+++ b/packages/google-auth/tests/test_agent_identity_utils.py
@@ -21,9 +21,7 @@
 from cryptography import x509
 import pytest
 
-from google.auth import _agent_identity_utils
-from google.auth import environment_vars
-from google.auth import exceptions
+from google.auth import _agent_identity_utils, environment_vars, exceptions
 
 # A mock PEM-encoded certificate without an Agent Identity SPIFFE ID.
 NON_AGENT_IDENTITY_CERT_BYTES = (
@@ -60,15 +58,22 @@ def test__is_agent_identity_certificate_invalid(self):
         cert = _agent_identity_utils.parse_certificate(NON_AGENT_IDENTITY_CERT_BYTES)
         assert not _agent_identity_utils._is_agent_identity_certificate(cert)
 
-    def test__is_agent_identity_certificate_valid_spiffe(self):
+    @pytest.mark.parametrize(
+        "spiffe_id",
+        [
+            "spiffe://agents.global.proj-12345.system.id.goog/workload",
+            "spiffe://agents.global.org-12345.system.id.goog/workload",
+            "spiffe://agents-nonprod.global.proj-12345.system.id.goog/workload",
+            "spiffe://agents-nonprod.global.org-12345.system.id.goog/workload",
+        ],
+    )
+    def test__is_agent_identity_certificate_valid_spiffe(self, spiffe_id):
         mock_cert = mock.MagicMock()
         mock_ext = mock.MagicMock()
         mock_san_value = mock.MagicMock()
         mock_cert.extensions.get_extension_for_oid.return_value = mock_ext
         mock_ext.value = mock_san_value
-        mock_san_value.get_values_for_type.return_value = [
-            "spiffe://agents.global.proj-12345.system.id.goog/workload"
-        ]
+        mock_san_value.get_values_for_type.return_value = [spiffe_id]
         assert _agent_identity_utils._is_agent_identity_certificate(mock_cert)
 
     def test__is_agent_identity_certificate_non_matching_spiffe(self):