diff --git a/packages/react-components/react-breadcrumb/cypress.config.ts b/packages/react-components/react-breadcrumb/cypress.config.ts
new file mode 100644
index 00000000000000..ca52cf041bbf2c
--- /dev/null
+++ b/packages/react-components/react-breadcrumb/cypress.config.ts
@@ -0,0 +1,3 @@
+import { baseConfig } from '@fluentui/scripts-cypress';
+
+export default baseConfig;
diff --git a/packages/react-components/react-breadcrumb/docs/Spec.md b/packages/react-components/react-breadcrumb/docs/Spec.md
index e49373e5993d8a..e340a0ef4d3178 100644
--- a/packages/react-components/react-breadcrumb/docs/Spec.md
+++ b/packages/react-components/react-breadcrumb/docs/Spec.md
@@ -176,6 +176,7 @@ Dropdown contains collapsed items.
 | ------------ | -------------------------- | ------------- | -------------------------------- |
 | appearance   | `transparent`, `subtle`    | `transparent` | Sets appearance                  |
 | dividerType  | `chevron`, `slash`         | `chevron`     | Sets type of divider             |
+| focusMode    | `tab`, `arrow`             | `tab`         | Sets focus mode                  |
 | iconPosition | `before`, `after`          | `before`      | Sets icon position for all items |
 | size         | `small`, `medium`, `large` | `medium`      | Defines size of the Breadcrumb   |
 
diff --git a/packages/react-components/react-breadcrumb/etc/react-breadcrumb.api.md b/packages/react-components/react-breadcrumb/etc/react-breadcrumb.api.md
index 486f705ec9af82..a6169e5317cced 100644
--- a/packages/react-components/react-breadcrumb/etc/react-breadcrumb.api.md
+++ b/packages/react-components/react-breadcrumb/etc/react-breadcrumb.api.md
@@ -105,7 +105,7 @@ export type BreadcrumbLinkState = ComponentState<BreadcrumbLinkSlots> & Partial<
 // @public
 export type BreadcrumbProps = ComponentProps<BreadcrumbSlots> & {
     appearance?: 'transparent' | 'subtle';
-    disableFocus?: boolean;
+    focusMode?: 'arrow' | 'tab';
     dividerType?: 'chevron' | 'slash';
     iconPosition?: 'before' | 'after';
     size?: 'small' | 'medium' | 'large';
diff --git a/packages/react-components/react-breadcrumb/package.json b/packages/react-components/react-breadcrumb/package.json
index 707fe49009ddd6..d6b2173d077069 100644
--- a/packages/react-components/react-breadcrumb/package.json
+++ b/packages/react-components/react-breadcrumb/package.json
@@ -16,6 +16,8 @@
     "build": "just-scripts build",
     "clean": "just-scripts clean",
     "code-style": "just-scripts code-style",
+    "e2e": "cypress run --component",
+    "e2e:local": "cypress open --component",
     "just": "just-scripts",
     "lint": "just-scripts lint",
     "test": "jest --passWithNoTests",
@@ -30,6 +32,7 @@
     "@fluentui/react-conformance": "*",
     "@fluentui/react-conformance-griffel": "9.0.0-beta.22",
     "@fluentui/scripts-api-extractor": "*",
+    "@fluentui/scripts-cypress": "*",
     "@fluentui/scripts-tasks": "*"
   },
   "dependencies": {
diff --git a/packages/react-components/react-breadcrumb/src/components/Breadcrumb/Breadcrumb.cy.tsx b/packages/react-components/react-breadcrumb/src/components/Breadcrumb/Breadcrumb.cy.tsx
new file mode 100644
index 00000000000000..fae1df3bb3bce1
--- /dev/null
+++ b/packages/react-components/react-breadcrumb/src/components/Breadcrumb/Breadcrumb.cy.tsx
@@ -0,0 +1,183 @@
+import * as React from 'react';
+import { mount } from '@cypress/react';
+import type {} from '@cypress/react';
+import { FluentProvider } from '@fluentui/react-provider';
+import { webLightTheme } from '@fluentui/react-theme';
+import { Breadcrumb, BreadcrumbItem, BreadcrumbButton, BreadcrumbLink } from '@fluentui/react-breadcrumb';
+import type { BreadcrumbProps } from '@fluentui/react-breadcrumb';
+
+const mountFluent = (element: JSX.Element) => {
+  mount(<FluentProvider theme={webLightTheme}>{element}</FluentProvider>);
+};
+
+const BreadcrumbSampleWithButton = (props: BreadcrumbProps) => (
+  <>
+    <p tabIndex={0} id="before">
+      Before
+    </p>
+
+    <Breadcrumb {...props} id="breadcrumb">
+      <BreadcrumbItem>
+        <BreadcrumbButton id="breadcrumb-button-1">Item 1</BreadcrumbButton>
+      </BreadcrumbItem>
+      <BreadcrumbItem>
+        <BreadcrumbButton id="breadcrumb-button-2">Item 2</BreadcrumbButton>
+      </BreadcrumbItem>
+      <BreadcrumbItem>
+        <BreadcrumbButton id="breadcrumb-button-3" current>
+          Item 3
+        </BreadcrumbButton>
+      </BreadcrumbItem>
+    </Breadcrumb>
+
+    <p tabIndex={0} id="after">
+      After
+    </p>
+  </>
+);
+
+const BreadcrumbSampleWithLink = (props: BreadcrumbProps) => (
+  <>
+    <p tabIndex={0} id="before">
+      Before
+    </p>
+
+    <Breadcrumb {...props} id="breadcrumb">
+      <BreadcrumbItem>
+        <BreadcrumbLink href="#" id="breadcrumb-link-1">
+          Item 1
+        </BreadcrumbLink>
+      </BreadcrumbItem>
+      <BreadcrumbItem>
+        <BreadcrumbLink href="#" id="breadcrumb-link-2">
+          Item 2
+        </BreadcrumbLink>
+      </BreadcrumbItem>
+      <BreadcrumbItem>
+        <BreadcrumbLink href="#" id="breadcrumb-link-3" current>
+          Item 3
+        </BreadcrumbLink>
+      </BreadcrumbItem>
+    </Breadcrumb>
+
+    <p tabIndex={0} id="after">
+      After
+    </p>
+  </>
+);
+
+const NonInteractiveBreadcrumbSample = (props: BreadcrumbProps) => (
+  <>
+    <p tabIndex={0} id="before">
+      Before
+    </p>
+
+    <Breadcrumb {...props} id="breadcrumb">
+      <BreadcrumbItem id="breadcrumb-item-1">Item 1</BreadcrumbItem>
+      <BreadcrumbItem id="breadcrumb-item-2">Item 2</BreadcrumbItem>
+      <BreadcrumbItem id="breadcrumb-item-3" current>
+        Item 3
+      </BreadcrumbItem>
+    </Breadcrumb>
+
+    <p tabIndex={0} id="after">
+      After
+    </p>
+  </>
+);
+
+describe('Breadcrumb', () => {
+  describe('focus behaviors for BreadcrumbButton', () => {
+    describe('focusMode="tab"(default)', () => {
+      it('should be focusable', () => {
+        mountFluent(<BreadcrumbSampleWithButton />);
+
+        cy.get('#before').focus();
+
+        cy.get('#breadcrumb-button-1').should('not.be.focused');
+
+        cy.realPress('Tab');
+
+        cy.get('#breadcrumb-button-1').should('be.focused');
+        cy.realPress('Tab');
+        cy.get('#breadcrumb-button-2').should('be.focused');
+        cy.realPress('Tab');
+        cy.get('#breadcrumb-button-3').should('be.focused');
+      });
+    });
+
+    describe('focusMode="arrow"', () => {
+      it('should be focusable', () => {
+        mountFluent(<BreadcrumbSampleWithButton focusMode="arrow" />);
+
+        cy.get('#before').focus();
+
+        cy.get('#breadcrumb-button-1').should('not.be.focused');
+
+        cy.realPress('Tab');
+
+        cy.get('#breadcrumb-button-1').should('be.focused');
+        cy.realPress('ArrowRight');
+        cy.get('#breadcrumb-button-2').should('be.focused');
+        cy.realPress('ArrowRight');
+        cy.get('#breadcrumb-button-3').should('be.focused');
+        cy.realPress('ArrowRight');
+        cy.get('#breadcrumb-button-1').should('be.focused');
+      });
+    });
+  });
+  describe('focus behaviors for BreadcrumbLink', () => {
+    describe('focusMode="tab"(default)', () => {
+      it('should be focusable', () => {
+        mountFluent(<BreadcrumbSampleWithLink />);
+
+        cy.get('#before').focus();
+
+        cy.get('#breadcrumb-link-1').should('not.be.focused');
+
+        cy.realPress('Tab');
+
+        cy.get('#breadcrumb-link-1').should('be.focused');
+        cy.realPress('Tab');
+        cy.get('#breadcrumb-link-2').should('be.focused');
+        cy.realPress('Tab');
+        cy.get('#breadcrumb-link-3').should('be.focused');
+      });
+    });
+
+    describe('focusMode="arrow"', () => {
+      it('should be focusable', () => {
+        mountFluent(<BreadcrumbSampleWithLink focusMode="arrow" />);
+
+        cy.get('#before').focus();
+
+        cy.get('#breadcrumb-link-1').should('not.be.focused');
+
+        cy.realPress('Tab');
+
+        cy.get('#breadcrumb-link-1').should('be.focused');
+        cy.realPress('ArrowRight');
+        cy.get('#breadcrumb-link-2').should('be.focused');
+        cy.realPress('ArrowRight');
+        cy.get('#breadcrumb-link-3').should('be.focused');
+        cy.realPress('ArrowRight');
+        cy.get('#breadcrumb-link-1').should('be.focused');
+      });
+    });
+  });
+  describe('focus behaviors for BreadcrumbItem', () => {
+    it('should not be focusable', () => {
+      mountFluent(<NonInteractiveBreadcrumbSample />);
+
+      cy.get('#before').focus();
+
+      cy.get('#breadcrumb-item-1').should('not.be.focused');
+      cy.get('#before').should('be.focused');
+
+      cy.realPress('Tab');
+
+      cy.get('#breadcrumb-item-1').should('not.be.focused');
+      cy.get('#after').should('be.focused');
+    });
+  });
+});
diff --git a/packages/react-components/react-breadcrumb/src/components/Breadcrumb/Breadcrumb.test.tsx b/packages/react-components/react-breadcrumb/src/components/Breadcrumb/Breadcrumb.test.tsx
index 9f0cc13830edb8..a12d4346ecb4aa 100644
--- a/packages/react-components/react-breadcrumb/src/components/Breadcrumb/Breadcrumb.test.tsx
+++ b/packages/react-components/react-breadcrumb/src/components/Breadcrumb/Breadcrumb.test.tsx
@@ -28,7 +28,6 @@ describe('Breadcrumb', () => {
         <nav
           aria-label="breadcrumb"
           class="fui-Breadcrumb"
-          data-tabster="{\\"mover\\":{\\"cyclic\\":true,\\"direction\\":2,\\"memorizeCurrent\\":true}}"
         >
           <ol
             class="fui-Breadcrumb__list"
@@ -62,7 +61,6 @@ describe('Breadcrumb', () => {
         <nav
           aria-label="breadcrumb"
           class="fui-Breadcrumb"
-          data-tabster="{\\"mover\\":{\\"cyclic\\":true,\\"direction\\":2,\\"memorizeCurrent\\":true}}"
         >
           <ol
             class="fui-Breadcrumb__list"
@@ -102,7 +100,6 @@ describe('Breadcrumb', () => {
         <nav
           aria-label="breadcrumb"
           class="fui-Breadcrumb"
-          data-tabster="{\\"mover\\":{\\"cyclic\\":true,\\"direction\\":2,\\"memorizeCurrent\\":true}}"
         >
           <ol
             class="fui-Breadcrumb__list"
diff --git a/packages/react-components/react-breadcrumb/src/components/Breadcrumb/Breadcrumb.types.ts b/packages/react-components/react-breadcrumb/src/components/Breadcrumb/Breadcrumb.types.ts
index 59e859d6cc7679..1a8a1638e1e7e2 100644
--- a/packages/react-components/react-breadcrumb/src/components/Breadcrumb/Breadcrumb.types.ts
+++ b/packages/react-components/react-breadcrumb/src/components/Breadcrumb/Breadcrumb.types.ts
@@ -34,11 +34,18 @@ export type BreadcrumbProps = ComponentProps<BreadcrumbSlots> & {
   appearance?: 'transparent' | 'subtle';
 
   /**
-   * Makes Breadcrumb not focusable.
+   * Sets the focus behavior for the Breadcrumb.
    *
-   * @default false
+   * `tab`
+   * This behaviour will cycle through all elements inside of the Breadcrumb when pressing the Tab key and then release focus
+   * after the last inner element.
+   *
+   * `arrow`
+   * This behaviour will cycle through all elements inside of the Breadcrumb when pressing the Arrow key.
+   *
+   * @default 'tab'
    */
-  disableFocus?: boolean;
+  focusMode?: 'arrow' | 'tab';
 
   /**
    * Controls type of the divider.
diff --git a/packages/react-components/react-breadcrumb/src/components/Breadcrumb/useBreadcrumb.ts b/packages/react-components/react-breadcrumb/src/components/Breadcrumb/useBreadcrumb.ts
index d0d5628eb8ae5e..79407c091d1a38 100644
--- a/packages/react-components/react-breadcrumb/src/components/Breadcrumb/useBreadcrumb.ts
+++ b/packages/react-components/react-breadcrumb/src/components/Breadcrumb/useBreadcrumb.ts
@@ -15,7 +15,7 @@ import { useArrowNavigationGroup } from '@fluentui/react-tabster';
 export const useBreadcrumb_unstable = (props: BreadcrumbProps, ref: React.Ref<HTMLElement>): BreadcrumbState => {
   const {
     appearance = 'transparent',
-    disableFocus,
+    focusMode = 'tab',
     dividerType = 'chevron',
     iconPosition = 'before',
     size = 'medium',
@@ -37,7 +37,7 @@ export const useBreadcrumb_unstable = (props: BreadcrumbProps, ref: React.Ref<HT
     root: getNativeElementProps('nav', {
       ref,
       'aria-label': props['aria-label'] ?? 'breadcrumb',
-      ...(!disableFocus ? focusAttributes : {}),
+      ...(focusMode === 'arrow' ? focusAttributes : {}),
       ...rest,
     }),
     list: resolveShorthand(list, { required: true, defaultProps: { role: 'list' } }),
diff --git a/packages/react-components/react-breadcrumb/src/components/BreadcrumbDivider/BreadcrumbDivider.test.tsx b/packages/react-components/react-breadcrumb/src/components/BreadcrumbDivider/BreadcrumbDivider.test.tsx
index 18aa18e3071a74..8343ddc43bdcee 100644
--- a/packages/react-components/react-breadcrumb/src/components/BreadcrumbDivider/BreadcrumbDivider.test.tsx
+++ b/packages/react-components/react-breadcrumb/src/components/BreadcrumbDivider/BreadcrumbDivider.test.tsx
@@ -51,7 +51,6 @@ describe('BreadcrumbDivider', () => {
         <nav
           aria-label="breadcrumb"
           class="fui-Breadcrumb"
-          data-tabster="{\\"mover\\":{\\"cyclic\\":true,\\"direction\\":2,\\"memorizeCurrent\\":true}}"
         >
           <ol
             class="fui-Breadcrumb__list"
@@ -82,7 +81,6 @@ describe('BreadcrumbDivider', () => {
         <nav
           aria-label="breadcrumb"
           class="fui-Breadcrumb"
-          data-tabster="{\\"mover\\":{\\"cyclic\\":true,\\"direction\\":2,\\"memorizeCurrent\\":true}}"
         >
           <ol
             class="fui-Breadcrumb__list"
diff --git a/packages/react-components/react-breadcrumb/stories/Breadcrumb/BreadcrumbFocusMode.tsx b/packages/react-components/react-breadcrumb/stories/Breadcrumb/BreadcrumbFocusMode.tsx
new file mode 100644
index 00000000000000..ddbe10f4a1edcb
--- /dev/null
+++ b/packages/react-components/react-breadcrumb/stories/Breadcrumb/BreadcrumbFocusMode.tsx
@@ -0,0 +1,86 @@
+import * as React from 'react';
+import { Breadcrumb, BreadcrumbItem, BreadcrumbButton, BreadcrumbDivider } from '@fluentui/react-breadcrumb';
+import { bundleIcon, CalendarMonth20Filled, CalendarMonth20Regular, GridDots20Regular } from '@fluentui/react-icons';
+import { ButtonProps } from '@fluentui/react-button';
+
+const CalendarMonth = bundleIcon(CalendarMonth20Filled, CalendarMonth20Regular);
+
+type Item = {
+  key: number;
+  item?: string;
+  buttonProps?: {
+    'aria-label'?: string;
+    onClick?: () => void;
+    icon?: ButtonProps['icon'];
+    disabled?: boolean;
+    iconPosition?: 'before' | 'after';
+  };
+};
+
+const buttonItems: Item[] = [
+  {
+    key: 0,
+    item: 'Item 0',
+  },
+  {
+    key: 1,
+    buttonProps: {
+      icon: <CalendarMonth />,
+      'aria-label': 'Item 1',
+    },
+  },
+  {
+    key: 2,
+    item: 'Item 2',
+    buttonProps: {
+      icon: <GridDots20Regular />,
+    },
+  },
+  {
+    key: 3,
+    item: 'Item 3',
+  },
+  {
+    key: 4,
+    item: 'Item 4',
+    buttonProps: {
+      icon: <CalendarMonth />,
+      iconPosition: 'after',
+    },
+  },
+  {
+    key: 5,
+    item: 'Item 5',
+  },
+];
+
+function renderButton(el: Item, isLastItem: boolean = false) {
+  return (
+    <React.Fragment key={`${el.key}-button`}>
+      <BreadcrumbItem>
+        <BreadcrumbButton
+          {...el.buttonProps}
+          current={isLastItem}
+          onClick={isLastItem ? undefined : el.buttonProps?.onClick}
+        >
+          {el.item}
+        </BreadcrumbButton>
+      </BreadcrumbItem>
+      {!isLastItem && <BreadcrumbDivider />}
+    </React.Fragment>
+  );
+}
+export const BreadcrumbFocusMode = () => (
+  <>
+    <h2>`tab` - default</h2>
+    Navigation using `tab` key.
+    <Breadcrumb aria-label="Breadcrumb with `tab` focusMode">
+      {buttonItems.map(el => renderButton(el, el.key === buttonItems.length - 1))}
+    </Breadcrumb>
+    <h2>`arrow`</h2>
+    Navigation using arrow keys
+    <Breadcrumb aria-label="Breadcrumb with `arrow` focusMode" focusMode="arrow">
+      {buttonItems.map(el => renderButton(el, el.key === buttonItems.length - 1))}
+    </Breadcrumb>
+  </>
+);
diff --git a/packages/react-components/react-breadcrumb/stories/Breadcrumb/index.stories.tsx b/packages/react-components/react-breadcrumb/stories/Breadcrumb/index.stories.tsx
index e8f0fd5aad0f75..8a02b840e9e63f 100644
--- a/packages/react-components/react-breadcrumb/stories/Breadcrumb/index.stories.tsx
+++ b/packages/react-components/react-breadcrumb/stories/Breadcrumb/index.stories.tsx
@@ -5,6 +5,7 @@ import bestPracticesMd from './BreadcrumbBestPractices.md';
 
 export { Default } from './BreadcrumbDefault.stories';
 export { BreadcrumbWithOverflow } from './BreadcrumbWithOverflow.stories';
+export { BreadcrumbFocusMode } from './BreadcrumbFocusMode';
 
 export default {
   title: 'Preview Components/Breadcrumb',
diff --git a/packages/react-components/react-breadcrumb/tsconfig.cy.json b/packages/react-components/react-breadcrumb/tsconfig.cy.json
new file mode 100644
index 00000000000000..93a140885851da
--- /dev/null
+++ b/packages/react-components/react-breadcrumb/tsconfig.cy.json
@@ -0,0 +1,9 @@
+{
+  "extends": "./tsconfig.json",
+  "compilerOptions": {
+    "isolatedModules": false,
+    "types": ["node", "cypress", "cypress-storybook/cypress", "cypress-real-events"],
+    "lib": ["ES2019", "dom"]
+  },
+  "include": ["**/*.cy.ts", "**/*.cy.tsx"]
+}
diff --git a/packages/react-components/react-breadcrumb/tsconfig.json b/packages/react-components/react-breadcrumb/tsconfig.json
index 1941a041d46c19..1317f81620ca5e 100644
--- a/packages/react-components/react-breadcrumb/tsconfig.json
+++ b/packages/react-components/react-breadcrumb/tsconfig.json
@@ -20,6 +20,9 @@
     },
     {
       "path": "./.storybook/tsconfig.json"
+    },
+    {
+      "path": "./tsconfig.cy.json"
     }
   ]
 }
diff --git a/packages/react-components/react-breadcrumb/tsconfig.lib.json b/packages/react-components/react-breadcrumb/tsconfig.lib.json
index 6f90cf95c005bd..e17f808c039339 100644
--- a/packages/react-components/react-breadcrumb/tsconfig.lib.json
+++ b/packages/react-components/react-breadcrumb/tsconfig.lib.json
@@ -16,7 +16,9 @@
     "**/*.test.ts",
     "**/*.test.tsx",
     "**/*.stories.ts",
-    "**/*.stories.tsx"
+    "**/*.stories.tsx",
+    "**/*.cy.ts",
+    "**/*.cy.tsx"
   ],
   "include": ["./src/**/*.ts", "./src/**/*.tsx"]
 }