diff --git a/Jenkinsfile b/Jenkinsfile
index 6cb1ec0e6ee..6be37f3faa8 100644
--- a/Jenkinsfile
+++ b/Jenkinsfile
@@ -49,6 +49,9 @@ pipeline {
         skipDefaultCheckout()
         preserveStashes(buildCount: 7)
     }
+    environment {
+        STAN_NUM_THREADS = '4'
+    }
     stages {
         stage('Kill previous builds') {
             when {
@@ -181,6 +184,17 @@ pipeline {
                         runTestsWin("test/unit")
                     }
                 }
+                stage('Windows Threading tests') {
+                    agent { label 'windows' }
+                    steps {
+                        deleteDirWin()
+                        unstash 'MathSetup'
+                        bat "echo CXX=${env.CXX} -Werror > make/local"
+                        bat "echo CPPFLAGS+=-DSTAN_THREADS >> make/local"
+                        runTestsWin("test/unit -f thread")
+                        runTestsWin("test/unit -f map_rect")
+                    }
+                }
             }
         }
         stage('Always-run tests part 2') {