From e441b35edc99316fa0934e874b3d8729f8ff95c6 Mon Sep 17 00:00:00 2001 From: Suganya Sivagurunathan Date: Thu, 26 Jan 2023 11:09:32 -0500 Subject: [PATCH] Update README.md Corrected the voxel size --- 3d_monolayer/README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/3d_monolayer/README.md b/3d_monolayer/README.md index aa0f74754..787d9a8cc 100644 --- a/3d_monolayer/README.md +++ b/3d_monolayer/README.md @@ -23,7 +23,7 @@ * Fiji > Image > Show Info… (Ctrl + I) * Search for something like “Voxel size” or record this metadata when collecting your own images * The actual units do not matter, rather their relative proportion. The numbers are unitless and therefore the decimal place does not matter. - * For this example, the relative pixel spacing is 0.065 in x and y and 0.29 pixels in z. + * For this example, the relative pixel spacing is 0.26 in x and y and 0.29 pixels in z. 1. Create "rule criteria" to identify an image by its color/channel. For example, using the Metadata you just extracted - `Metadata -> Does -> Have ChannelNumber matching -> 0` would match the first image. 1. Give the images "variable names" that describe the contents in the image. For example, use the name *dna* or *dapi* to describe an image stained with DAPI.