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Feature/pass reads#361

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Adoni5 merged 9 commits into
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feature/pass-reads
Aug 26, 2024
Merged

Feature/pass reads#361
Adoni5 merged 9 commits into
mainfrom
feature/pass-reads

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@Adoni5

@Adoni5 Adoni5 commented Jun 19, 2024

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  1. Uses the pass_reads function on the caller, rather than individual calls to pass_read, should be faster and has removed sleeping.
  2. Tidies up the example reads in the explainer about sub_tag and makes them comments.
  3. Added a log statement about generating the aligner description, as it felt to me that after logging "starting main loop" it was hanging, when in actuality a large reference takes a while to generate a description for.

N.B Committed as @satriobio from the remote Vs code server, is this identity theft?

@Adoni5 Adoni5 mentioned this pull request Jun 19, 2024
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Adoni5 requested review from alexomics and mattloose July 8, 2024 14:48
mattloose
mattloose previously approved these changes Jul 9, 2024

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Looks good to me.

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This PR is stale because it has been open 45 days with no activity. Remove stale label or comment or this will be closed in 10 days.

@github-actions github-actions Bot added the Stale label Aug 24, 2024
* Made a proper SVG thought i'd just include it here

* Remove setting the read_number on the result
Add in the correct strand classifications for minknow 6

* Remove Read number from Result and all references to read number

* Update target versions for MinKNOW to 6.0.0 - 6.0.0
Explicitly exit if running on MInKNOW 5.X.X

* Update vendored read until API to v3.5.2

* Update test for conencted base caller version to work

* Pin ont-pybasecall-client-lib at 7.4.12

* Add downgrade to 2024.2.0 to error message

* Fix bug in reads not unblocking as expected #372

* change list() to []

* Chunk tracker uses read_id not read_number, update trackers internal variable names

* bump version to 2024.3.0

* Fix tests for the new MinKNOW version compatibility
Fix tests for the _statistics too not use `read_number`

* Bump summarise version to 0.2.7

* Drop incorrect gotcha

* Feature/deprecate guppy (#374)

* This commit addresses the removal of guppy.py, partial removal of guppy tests, edits to documentation to largely replace guppy with dorado, removes the ont-pyguppy-client-lib and adds a warning to the readme. It also adds some extra classes to the read_until init in targets.

* Remove builtin module for guppy

* Update FAQ questions to focus dorado as well as Guppy

* Change validate caller warning to mention dorado not guppy

* Change readme warnings to Focus dorado and minknow breaking changes more
Add link/requirement for dorado_basecall_server not guppy_basecall_server

* Rename tests to dorado validation tests

* Add dorado to mappy-test install target
alexomics
alexomics previously approved these changes Aug 26, 2024
@Adoni5

Adoni5 commented Aug 26, 2024

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Fixes #372 in 9ee6b8b, caused by extra clipping shenanigans of the base-called Results read-id. Was losing the first 3 UUID chars, so no read existed to unblock.

@Adoni5
Adoni5 merged commit 8c013a4 into main Aug 26, 2024
agrima2010 pushed a commit to agrima2010/readfish that referenced this pull request Apr 17, 2026
* Attempt at using pass_reads

* Add message about generating the aligner description

* FIX bug where parameter reads was being overwritten by empty list reads

* DRAFT Feature/minknow6 compatibility (LooseLab#369)

* Made a proper SVG thought i'd just include it here

* Remove setting the read_number on the result
Add in the correct strand classifications for minknow 6

* Remove Read number from Result and all references to read number

* Update target versions for MinKNOW to 6.0.0 - 6.0.0
Explicitly exit if running on MInKNOW 5.X.X

* Update vendored read until API to v3.5.2

* Update test for conencted base caller version to work

* Pin ont-pybasecall-client-lib at 7.4.12

* Add downgrade to 2024.2.0 to error message

* Fix bug where unblocked_read_ids wasn't being written out

* Chunk tracker uses read_id not read_number, update trackers internal variable names

* bump version to 2024.3.0

* Fix tests for the new MinKNOW version compatibility
Fix tests for the _statistics too not use `read_number`

* Bump summarise version to 0.2.7

* Drop incorrect gotcha

* Feature/deprecate guppy (LooseLab#374)

* This commit addresses the removal of guppy.py, partial removal of guppy tests, edits to documentation to largely replace guppy with dorado, removes the ont-pyguppy-client-lib and adds a warning to the readme. It also adds some extra classes to the read_until init in targets.

* Remove builtin module for guppy

* Update FAQ questions to focus dorado as well as Guppy

* Change validate caller warning to mention dorado not guppy

* Change readme warnings to Focus dorado and minknow breaking changes more
Add link/requirement for dorado_basecall_server not guppy_basecall_server

* Rename tests to dorado validation tests

* Add dorado to mappy-test install target

* Fix black formatting of accepted chunks list

* rename read_id to read_number on _generate_action
because I may as well

* return action from override as for some pythons it doesn't mutate in place

* add mappy-rs because I keep typing it and not mappy_rs

* Don't clip RF from front, used cached read_id for the result
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4 participants