basecall problem

[yuanzhao0502]

I have a ab1 file tracy basecall only generate 121 bases but it visualized normal after that. What's the reason tracy can not doing the basecalling after that? Thank you for help!

zip format of ab1 file

[tobiasrausch]

Tracy requires the original basecall positions from the Applied Biosystem machine. I guess in your case the machine terminated the Autoanalysis prematurely and thus, I would suggest to re-run the sequencing analysis on the machine.

[yuanzhao0502]

Tracy requires the original basecall positions from the Applied Biosystem machine. I guess in your case the machine terminated the Autoanalysis prematurely and thus, I would suggest to re-run the sequencing analysis on the machine.

Thanks a lot for your suggest!

Actually I have a try to use biopython to read this file. "record.letter_annotations" can identify 972 bases. What's the differences of basecalling method between tracy and biopython?