Gene Volcano Plot Web App

This Flask-based web application generates an interactive volcano plot and box plots using data from an Excel file.

Prerequisites

Requirements:

• Python (>=3.8)
• pip
• Miniconda or Anaconda (optional, for virtual environment management)

Required Python Packages

In your conda virtual environment(suggested):

conda create -n flEnv
conda activate flEnv

Install dependencies by running:

pip install flask pandas numpy matplotlib plotly openpyxl

or if using conda:

conda install -c conda-forge flask pandas numpy matplotlib plotly openpyxl

Running the Application

1. Clone or download the repository containing this project.
2. Navigate to the project directory:

cd /path/to/project

3. Start the Flask app:

python app.py

Alternatively (from app.py level):

flask run

4. Open your browser and go to:

http://127.0.0.1:5000/plot

Features

• Volcano Plot: Displays a scatter plot of log fold change vs. adjusted p-value.
• Box Plot: Allows visual comparison of protein concentrations for specific genes across young and old donors.
• Interactive UI: Hovering over points reveals gene information.