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biosequence-analyzer-api

Overview

biosequence-analyzer-api is a REST API built with FastAPI and Biopython for DNA and RNA sequence analysis. It provides core bioinformatics utilities such as sequence transformation, GC content calculation, reverse complement generation, transcription, translation, and FASTA parsing.

The project is designed as a backend-only service intended for integration into other applications, pipelines, or bioinformatics tools.

Features

  • DNA/RNA sequence analysis
  • GC content calculation
  • Reverse complement generation
  • DNA transcription (DNA → RNA)
  • RNA/DNA translation (RNA → protein)
  • FASTA file parsing support
  • RESTful API design for easy integration

Tech Stack

  • Python
  • FastAPI
  • Biopython
  • Uvicorn (ASGI server)

Project Structure

app/ main.py # Application entry point routes/ # API route definitions services/ # Core sequence processing logic schemas/ # Request and response models

Installation

1. Clone the repository

git clone https://github.com/your-username/biosequence-analyzer-api.git
cd biosequence-analyzer-api

2. Create virtual environment

python -m venv venv
source venv/bin/activate  # Linux/Mac
venv\Scripts\activate     # Windows

3. Install dependencies

pip install -r requirements.txt

Running the API

Start the development server:

uvicorn app.main:app --reload

The API will be available at:

http://127.0.0.1:8000

Interactive API documentation:

http://127.0.0.1:8000/docs

API Endpoints

Analyze Sequence

POST /analyze

Request body:

{
  "sequence": "ATGCATGC"
}

Response:

{
  "gc_content": 50.0,
  "reverse_complement": "GCATGCAT",
  "rna": "AUGCAUGC",
  "protein": "MHA"
}

Planned Improvements

  • FASTA file upload support
  • Batch sequence processing
  • Authentication layer (optional)
  • Rate limiting
  • Docker support
  • Cloud deployment configuration

Use Cases

  • Bioinformatics research tools
  • Educational platforms for molecular biology
  • Backend service for genomics applications
  • Integration into scientific data pipelines

License

MIT License

Notes

This project focuses on backend processing and does not include a frontend. It is intended to be consumed as a standalone API service.

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FastAPI-based REST API for DNA/RNA sequence analysis powered by Biopython. Supports sequence transformation, GC content, and FASTA parsing.

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